Improved Murine Blastocyst Quality and Development in a Single Culture Medium Compared to Sequential Culture Media
نویسندگان
چکیده
منابع مشابه
overcoming to blastocyst collapse and improvement of blastocyst development in sequential culture media
introduction: this study was started to improve in vitro blastocyst development and avoiding of blastocyst collapsment (no visibie cavity, returning of hatching blastocyst into zona pellucida, and tendency to degeneration). materials and methods: 24hrs one - cell mouse embryos were recovered from positive plug females and cultured in following canditions: experiments 1,2: during first 48hrs nmr...
متن کاملImproved methods for blastocyst formation and culture.
Transfer at the blastocyst stage has been proposed to increase the pregnancy rates after in-vitro fertilization (IVF) and embryo transfer. In the first period of experiments, culture in a single medium, from fertilization to blastocyst led to disappointing results: low blastocyst formation rates and low implantation rates per blastocyst transferred. Then the period of co-culture began (starting...
متن کاملTime to switch from co-culture to sequential defined media for transfer at the blastocyst stage.
It is current practice in in-vitro fertilization (IVF) to transfer embryos 48 h post-insemination at the 2–4 cell stage. However, it is now clear that this is a totally blind process. Firstly, embryos encounter an unsuitable environment, normally they would be in the oviduct. Secondly, motility of the uterus is related inversely to circulating progesterone. This problem of hypermotility of the ...
متن کاملA single medium and sequential media are equally effective for the culture of human embryos to the blastocyst stage: a pilot study
Ovulation Induction and Oocyte Recovery Ovarian hyperstimulation was achieved using GnRH analogue (nafarelin acetate, Synarel®, Pfizer) along with human recombinant FSH (r-hFSH: Gonal-F®, MerckSerono) in a long down-regulation protocol. Starting gonadotrophin dose varied from 150-300 IU and was based on female age, body mass index, ovarian volume, basal FSH levels and the number of preantral fo...
متن کاملImproved development of rabbit one-cell embryos to the hatching blastocyst stage by culture in a defined, protein-free culture medium.
In Exp. 1, Medium 199 and Medium RD (RPMI-1640 and Dulbecco's MEM, 1:1 v/v) were compared in a 2 x 2 factorial design by supplementing each with 15 mg bovine serum albumin (BSA)/ml of 1 mg polyvinyl alcohol (PVA)/ml. All media contained 5 micrograms insulin/ml, 5 micrograms transferrin/ml, 5 ng selenium/ml (ITS), and 10 ng epidermal growth factor (EGF)/ml. One-cell embryos were cultured at 39 d...
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ژورنال
عنوان ژورنال: Reproductive Sciences
سال: 2015
ISSN: 1933-7191,1933-7205
DOI: 10.1177/1933719115618281